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Protocols

Selected Vector kit instructions and associated protocols are listed below in PDF format. If you are unable to obtain the desired information please contact Vector technical service or your local Vector Labs distributor for further assistance.

Test Attachs (PK-8501)
Lab Restart Checklist
Folio3 Tests (P-9002,Folio3 Test)
Test Attacment3 (Test Redirect 2)
Test Attacment33 (Test Redirect 2)

Bioconjugation Reagents

User Guide (MB-9002) (MB-9002)
Test Attach1 (SP-2002)
S-9011 Product Manual (S-9011)
A-9202 Product Manual (A-9202)

Supplemental Protocols for Vector® Kits and Reagents

Human on Human Immunodetection (User Guide) (HOH-3000)
Enzyme Immunoassays, Hybridoma Screening, and Western Blots (AK-5000,AK-5001,AK-5002,AK-5004,AK-5200,PK-4000,PK-4002,PK-4004,PK-4005,PK-4007,PK-4010,PK-6100,PK-6101,PK-6102,PK-6103,PK-6104,PK-6105,PK-6106,PK-6200,PK-7100,PK-7200)
BLOXALL® Solution for Quenching Endogenous Peroxidase and Alkaline Phosphatase Activity (SP-6000-100)
Recommended Dilutions and Buffers
Troubleshooting Guide (AK-5000,AK-5001,AK-5002,AK-5004,AK-5200,PK-4000,PK-4002,PK-4004,PK-4005,PK-4007,PK-4010,PK-6100,PK-6101,PK-6102,PK-6103,PK-6104,PK-6105,PK-6106,PK-6200,PK-7100,PK-7200)
Enzyme Substrate Compatibility Chart (SK-4103-400,SK-4105,SK-4200,SK-4205,SK-4285,SK-4600,SK-4605,SK-4700,SK-4705,SK-4800,SK-4805,SK-5100,SK-5105,SK-5200,SK-5300,SK-5400,SK-4100,SK-4400)

Mounting Media

VECTASHIELD PLUS Antifade Mounting Medium (User Guide) (H-1900)
VECTASHIELD PLUS Antifade Mounting Medium with DAPI (User Guide) (H-2000)
VECTASHIELD® Vibrance Antifade Mounting Medium (H-1700)
VECTASHIELD® Vibrance Antifade Mounting Medium with DAPI (H-1800)
VectaMount® Permanent Mounting Medium (H-5000-60)
VectaMount® AQ Aqueous Mounting Medium (H-5501-60)
VECTASHIELD® Mounting Media (H-1000-10)
VECTASHIELD® Mounting Media with DAPI (H-1200-10)
VECTASHIELD® Mounting Media with PI (H-1300-10)
VECTASHIELD® HardSet Mounting Media (H-1400-10)
VECTASHIELD® HardSet Mounting Media with DAPI (H-1500-10)

VECTASTAIN® Kits

VECTASTAIN® Elite ABC-Peroxidase Kit (PK-6100,PK-6101,PK-6102,PK-6103,PK-6104,PK-6105,PK-6106)
VECTASTAIN® Universal Elite ABC-Peroxidase Kit (PK-6200)
R.T.U. VECTASTAIN® Elite ABC-Peroxidase Reagent (PK-7100)
R.T.U. VECTASTAIN® Universal Elite ABC-Peroxidase Kit (PK-7200)
VECTASTAIN® ABC-Peroxidase Kit (PK-4000)
VECTASTAIN® ABC-Alkaline Phosphatase Kit (AK-5000)
VECTASTAIN® Universal ABC-Alkaline Phosphatase Kit (AK-5200)
VECTASTAIN® Universal Quick Kit (PK-8800)
R.T.U. VECTASTAIN® Universal Quick Kit (PK-7800)

Vector Mouse on Mouse (M.O.M.®) Immunodetection Kit

Vector® Mouse on Mouse (M.O.M.®) Elite Peroxidase Immunodetection Kit (PK-2200)
Vector® Mouse on Mouse (M.O.M.®) Fluorescein Immunodetection Kit (FMK-2201)
Vector® Mouse on Mouse (M.O.M.®) Basic Immunodetection Kit (BMK-2202)
Multiple Immunofluorescent Staining Using Mouse Antibodies (BMK-2202,FMK-2201)
Troubleshooting Mouse Primaries on Mouse Tissue (BMK-2202,FMK-2201,MP-2400,PK-2200)
Multiple Antigen Labeling in the Same Tissue Sections (AK-5000,AK-5001,AK-5002,AK-5004,AK-5200,BMK-2202,FMK-2201,MP-2400,MP-7404-50,MP-7422-15,MP-7444-15,PK-2200,PK-4000,PK-4002,PK-4004,PK-4005,PK-4007,PK-4010,PK-6100,PK-6101,PK-6102,PK-6103,PK-6104,PK-6105,PK-6106,PK-6200,PK-7100,PK-7200,SK-4103-400,SK-4105,SK-4200,SK-4205,SK-4285,SK-4600,SK-4605,SK-4700,SK-4705,SK-4800,SK-4805,SK-5100,SK-5105,SK-5200,SK-5300,SK-5400,SK-4100,SK-4400,MP-7401,MP-7402,MP-7500,MP-5401,MP-5402,MP-7405,MP-7451,MP-7452,MP-6401-15,MP-6402-15,MP-7601,MP-7602,MP-5404-15,MP-5405,MP-5444-15)

Immunofluorescence

TrueVIEW Autofluorescence Quenching Kit (SP-8400-15)
TrueVIEW®?Autofluorescence Quenching Kit with DAPI (SP-8500-15)
Double Immunofluorescent Labeling (A-2011-1,A-2016-1)
VectaFluor® DyLight® 488 Anti-Goat Ig (DI-3788-15)
VectaFluor® DyLight® 488 Anti-Mouse Ig (DI-2788-15)
VectaFluor® DyLight® 488 Anti-Rabbit Ig (DI-1788-15)
VectaFluor® DyLight® 594 Anti-Goat Ig (DI-3794-15)
VectaFluor® DyLight® 594 Anti-Mouse Ig (DI-2794-15)
VectaFluor® DyLight® 594 Anti-Rabbit Ig (DI-1794-15)
VectaFluor® Excel Amplified DyLight® 488 Anti-Mouse Ig (DK-2488)
VectaFluor® Excel Amplified DyLight® 488 Anti-Rabbit Ig (DK-1488)
VectaFluor® Excel Amplified DyLight® 594 Anti-Mouse Ig (DK-2594)
VectaFluor® Excel Amplified DyLight® 594 Anti-Rabbit Ig (DK-1594)
VECTABOND® Reagent for Tissue Section Adhesion (SP-1800-7)
Antigen Unmasking Solution (H-3300-250,H-3301-250)
Avidin/Biotin Blocking Kit (SP-2001)
Streptavidin/Biotin Blocking Kit (SP-2002)

ImmPRESS® Polymerized Reporter Enzyme Staining System

ImmPRESS®-AP Anti-Rabbit Ig (alkaline phosphatase) (MP-5401)
ImmPRESS®-AP Anti-Mouse Ig (alkaline phosphatase) (MP-5402)
Vector® Mouse on Mouse (M.O.M.®) ImmPRESS Peroxidase Polymer Immunodetection Kit (MP-2400)
ImmPRESS® Anti-Rabbit Ig (peroxidase) (MP-7401)
ImmPRESS® Anti-Mouse Ig (peroxidase) (MP-7402)
ImmPRESS® Anti-Mouse Ig, Rat-adsorbed (peroxidase) (MP-7422-15)
ImmPRESS® Anti-Rat Ig (peroxidase) (MP-7404-50)
ImmPRESS® Anti-Rat Ig, Mouse-adsorbed (peroxidase) (MP-7444-15)
ImmPRESS® Anti-Goat Ig (peroxidase) (MP-7405)
ImmPRESS® Universal Antibody Kit (peroxidase) (MP-7500)
ImmPRESS® Excel Anti-Rabbit Ig (peroxidase, complete) (MP-7601)
ImmPRESS® Excel Anti-Mouse Ig (peroxidase, complete) (MP-7602)

Counterstains

Vector® Hematoxylin (H-3401-500)
Vector® Hematoxylin QS (H-3404-100)
Vector® Methyl Green (H-3402-500)
Vector® Nuclear Fast Red (H-3403-500)
Counterstain/Substrate Compatibility Chart (H-3401-500,H-3402-500,H-3403-500,H-3404-100,SK-4103-400,SK-4105,SK-4200,SK-4205,SK-4285,SK-4600,SK-4605,SK-4700,SK-4705,SK-4800,SK-4805,SK-5100,SK-5105,SK-5200,SK-5300,SK-5400,SK-4100,SK-4400)

Enzyme Substrates

ImmPACT® DAB Substrate Kit (SK-4105)
ImmPACT® DAB EqV Substrate Kit (SK-4103-400)
ImmPACT® AEC Substrate Kit (SK-4205)
ImmPACT® AMEC Red Substrate Kit (SK-4285)
ImmPACT® VIP Substrate Kit (SK-4605)
ImmPACT® SG Substrate Kit (SK-4705)
ImmPACT® NovaRED Substrate Kit (SK-4805)
DAB Substrate Kit (SK-4100)
DAB Enhancing Solution (H-2200-30)
Vector® VIP Substrate Kit (SK-4600)
Vector® SG Substrate Kit (SK-4700)
Vector® NovaRED Substrate Kit (SK-4800)
AEC Substrate Kit (SK-4200)
TMB Substrate Kit (SK-4400)
DuoLuX® Chemiluminescent/Fluorescent Substrate Kit for peroxidase (SK-6604-200)
ImmPACT® Vector® Red Substrate Kit (SK-5105)
Vector® Red Substrate Kit (SK-5100)
Vector® Blue Substrate Kit (SK-5300)
Vector® Black Substrate Kit (SK-5200)
BCIP/NBT Substrate Kit (SK-5400)
Levamisole Solution (SP-5000-18)
p-Nitrophenyl phosphate Substrate Kit (SK-5900-18)
DuoLuX® Chemiluminescent/Fluorescent Substrate Kit for alkaline phosphatase (SK-6605-100)

Blot Detection Systems

WestVision® Peroxidase Polymer Reagents for Western Blotting (WB-1000-.8,WB-2000-.8)
VECTASTAIN® ABC-AmP® Western Blot Detection System (AK-6000)
Troubleshooting Guide for VECTASTAIN® ABC-AmP® Kits (AK-6000)

In Situ Hybridization

Fluorescence Detection of Biotin-Labeled ISH Probes (A-2011-1,BA-0300-.5)
Fluorescence Detection of Fluorescein-Labeled ISH Probes (A-2011-1,BA-0601-.5)
Chromogenic Detection of Biotin-Labeled ISH Probes (SA-5100-1)
In Situ Hybridization Detection Systems (A-2011-1,BA-0300-.5,BA-0601-.5,H-1000-10,H-1200-10,H-1300-10,H-1400-10,H-1500-10,H-5000-60,H-5501-60,MB-1220-100,SA-5100-1,SK-5400,SP-5000-18,H-1700,H-1800)
Amplification of FISH using Biotinylated Anti-Avidin (BA-0300-.5)

Biotin Labeling Reagents

Biotin (Long Arm) NHS (SP-1200-50)
Biotin (Long Arm) NHS, water soluble (SP-1210-50)
Biotin (Long Arm) Maleimide (SP-1501-12)
Biotin (Long Arm) Hydrazide (SP-1100-50)

Biotin Quantitation Kit

Quant*Tag Biotin Kit (BDK-2000)

Thiol-Reactive Labeling Reagents

Biotin Maleimide (SP-1501-12)
Fluorescein Maleimide (SP-1502-12)
Texas Red Maleimide (SP-1505-3.6)

Molecular Biology Reagents

5' EndTag Labeling System (MB-9001)
3' EndTag Labeling System (MB-9002)
PHOTOPROBE® Biotin (SP-1000-.5,SP-1020-.5)
Detection of Labeled Nucleic Acid Probes on Blots (H-3401-500,H-3402-500,H-3403-500,H-3404-100,SK-4103-400,SK-4105,SK-4200,SK-4205,SK-4285,SK-4600,SK-4605,SK-4700,SK-4705,SK-4800,SK-4805,SK-5100,SK-5105,SK-5200,SK-5300,SK-5400,SK-4100,SK-4400)
NicKit® Probe Size Optimization Kit (MB-1905)

Neuronal Tracing reagents

PHA-L (L-1110-5)
NEUROBIOTIN® Tracer (SP-1120)

Lectins

Lectins in Histochemistry, ELISA, and Western Blot Applications (AL-1013-2,AL-1063-2,AL-1143-2,AL-1173-2,AL-1183-2,AL-1213-2,AL-1233-2,AL-1303-2,AL-1353-2,AL-1393-2,B-1005-5,B-1005S-5,B-1015-5,B-1025-5,B-1025S-5,B-1035-5,B-1045-5,B-1055-5,B-1065-2,B-1075-5,B-1085-5,B-1105-2,B-1115-2,B-1125-2,B-1145-5,B-1155-5,B-1165-2,B-1175-1,B-1185-2,B-1205-.5,B-1215-2,B-1235-2,B-1245-2,B-1265-1,B-1305-2,B-1315-2,B-1325-2,B-1345-2,B-1355-2,B-1375-2,B-1385-2,B-1395-1,BK-1000,BK-3000,DL-1174-1,DL-1177-1,DL-1207-.5,FL-1001-25,FL-1011-2,FL-1021S-5,FL-1041-5,FL-1081-5,FL-1111-2,FL-1121-2,FL-1141-5,FL-1151-5,FL-1161-2,FL-1171-1,FL-1181-2,FL-1201-.5,FL-1211-2,FL-1231-2,FL-1241-2,FL-1301-2,FL-1311-2,FL-1321-2,FL-1341-2,FL-1351-2,FL-1391-1,FLK-2100,FLK-4100,L-1000-500,L-1020S-10,L-1050-10,L-1080-10,L-1090-10,L-1100-5,L-1110-5,L-1120-5,L-1140-10,L-1160-5,L-1170-2,L-1180-5,L-1190-1,L-1210-5,L-1230-5,L-1240-5,L-1260-2,L-1280-5,L-1290-1,L-1300-5,L-1310-5,L-1320-5,L-1340-5,L-1350-5,L-1370-5,L-1380-5,L-1390-2,L-1104-1,L-1410-5,DL-1178-1,AL-1003,AL-1023,AL-1023S,AL-1043,AL-1073,AL-1083,AL-1153,AL-1243,FL-1021,FL-1031,FL-1061,FL-1071,FL-1101,L-1010,L-1020,L-1030,L-1040,L-1060,L-1070,L-1150,DL-1048-1,L-1420-2,DL-1208-.5,DL-1067-1,DL-1068-1,CL-1003-1,CL-1073-1,CL-1075-1,CL-1303-1,CL-1305-1,BK-2100)
Peroxidase localization of WGA in Neural Tissue (L-1020)
Agarose WGA - Use and Regeneration (AL-1023)
Enrichment of NK cells by panning on ECL plates (L-1140-10)
Specificity of Jacalin binding (L-1150)