Protocols
Selected Vector kit instructions and associated protocols are listed below in PDF format. If you are unable to obtain the desired information please contact Vector technical service or your local Vector Labs distributor for further assistance.
Test Attachs
(PK-8501)
Folio3 Tests
(P-9002,Folio3 Test)
Test Attacment3
(Test Redirect 2)
Test Attacment33
(Test Redirect 2)
Bioconjugation Reagents
User Guide (MB-9002)
(MB-9002)
Test Attach1
(SP-2002)
S-9011 Product Manual
(S-9011)
A-9202 Product Manual
(A-9202)
Supplemental Protocols for Vector® Kits and Reagents
Human on Human Immunodetection (User Guide)
(HOH-3000)
Enzyme Immunoassays, Hybridoma Screening, and Western Blots
(AK-5000,AK-5001,AK-5002,AK-5004,AK-5200,PK-4000,PK-4002,PK-4004,PK-4005,PK-4007,PK-4010,PK-6100,PK-6101,PK-6102,PK-6103,PK-6104,PK-6105,PK-6106,PK-6200,PK-7100,PK-7200)
BLOXALL® Solution for Quenching Endogenous Peroxidase and Alkaline Phosphatase Activity
(SP-6000-100)
Troubleshooting Guide
(AK-5000,AK-5001,AK-5002,AK-5004,AK-5200,PK-4000,PK-4002,PK-4004,PK-4005,PK-4007,PK-4010,PK-6100,PK-6101,PK-6102,PK-6103,PK-6104,PK-6105,PK-6106,PK-6200,PK-7100,PK-7200)
Enzyme Substrate Compatibility Chart
(SK-4103-400,SK-4105,SK-4200,SK-4205,SK-4285,SK-4600,SK-4605,SK-4700,SK-4705,SK-4800,SK-4805,SK-5100,SK-5105,SK-5200,SK-5300,SK-5400,SK-4100,SK-4400)
Mounting Media
VectaMount® Permanent Mounting Medium
(H-5000-60)
VectaMount® AQ Aqueous Mounting Medium
(H-5501-60)
VECTASHIELD® Mounting Media
(H-1000-10)
VECTASHIELD® Mounting Media with DAPI
(H-1200-10)
VECTASHIELD® Mounting Media with PI
(H-1300-10)
VECTASHIELD® HardSet Mounting Media
(H-1400-10)
VECTASHIELD® HardSet Mounting Media with DAPI
(H-1500-10)
VECTASTAIN® Kits
VECTASTAIN® Elite ABC-Peroxidase Kit
(PK-6100,PK-6101,PK-6102,PK-6103,PK-6104,PK-6105,PK-6106)
VECTASTAIN® ABC-Peroxidase Kit
(PK-4000)
VECTASTAIN® ABC-Alkaline Phosphatase Kit
(AK-5000)
VECTASTAIN® Universal Quick Kit
(PK-8800)
R.T.U. VECTASTAIN® Universal Quick Kit
(PK-7800)
Vector Mouse on Mouse (M.O.M.®) Immunodetection Kit
Multiple Immunofluorescent Staining Using Mouse Antibodies
(BMK-2202,FMK-2201)
Troubleshooting Mouse Primaries on Mouse Tissue
(BMK-2202,FMK-2201,MP-2400,PK-2200)
Multiple Antigen Labeling in the Same Tissue Sections
(AK-5000,AK-5001,AK-5002,AK-5004,AK-5200,BMK-2202,FMK-2201,MP-2400,MP-7404-50,MP-7422-15,MP-7444-15,PK-2200,PK-4000,PK-4002,PK-4004,PK-4005,PK-4007,PK-4010,PK-6100,PK-6101,PK-6102,PK-6103,PK-6104,PK-6105,PK-6106,PK-6200,PK-7100,PK-7200,SK-4103-400,SK-4105,SK-4200,SK-4205,SK-4285,SK-4600,SK-4605,SK-4700,SK-4705,SK-4800,SK-4805,SK-5100,SK-5105,SK-5200,SK-5300,SK-5400,SK-4100,SK-4400,MP-7401,MP-7402,MP-7500,MP-5401,MP-5402,MP-7405,MP-7451,MP-7452,MP-6401-15,MP-6402-15,MP-7601,MP-7602,MP-5404-15,MP-5405,MP-5444-15)
Immunofluorescence
TrueVIEW Autofluorescence Quenching Kit
(SP-8400-15)
TrueVIEW®?Autofluorescence Quenching Kit with DAPI
(SP-8500-15)
Double Immunofluorescent Labeling
(A-2011-1,A-2016-1)
VectaFluor® DyLight® 488 Anti-Goat Ig
(DI-3788-15)
VectaFluor® DyLight® 488 Anti-Mouse Ig
(DI-2788-15)
VectaFluor® DyLight® 488 Anti-Rabbit Ig
(DI-1788-15)
VectaFluor® DyLight® 594 Anti-Goat Ig
(DI-3794-15)
VectaFluor® DyLight® 594 Anti-Mouse Ig
(DI-2794-15)
VectaFluor® DyLight® 594 Anti-Rabbit Ig
(DI-1794-15)
VECTABOND® Reagent for Tissue Section Adhesion
(SP-1800-7)
Antigen Unmasking Solution
(H-3300-250,H-3301-250)
Avidin/Biotin Blocking Kit
(SP-2001)
Streptavidin/Biotin Blocking Kit
(SP-2002)
ImmPRESS® Polymerized Reporter Enzyme Staining System
ImmPRESS® Anti-Rabbit Ig (peroxidase)
(MP-7401)
ImmPRESS® Anti-Mouse Ig (peroxidase)
(MP-7402)
ImmPRESS® Anti-Mouse Ig, Rat-adsorbed (peroxidase)
(MP-7422-15)
ImmPRESS® Anti-Rat Ig (peroxidase)
(MP-7404-50)
ImmPRESS® Anti-Rat Ig, Mouse-adsorbed (peroxidase)
(MP-7444-15)
ImmPRESS® Anti-Goat Ig (peroxidase)
(MP-7405)
Counterstains
Vector® Hematoxylin
(H-3401-500)
Vector® Hematoxylin QS
(H-3404-100)
Vector® Methyl Green
(H-3402-500)
Vector® Nuclear Fast Red
(H-3403-500)
Counterstain/Substrate Compatibility Chart
(H-3401-500,H-3402-500,H-3403-500,H-3404-100,SK-4103-400,SK-4105,SK-4200,SK-4205,SK-4285,SK-4600,SK-4605,SK-4700,SK-4705,SK-4800,SK-4805,SK-5100,SK-5105,SK-5200,SK-5300,SK-5400,SK-4100,SK-4400)
Enzyme Substrates
ImmPACT® DAB Substrate Kit
(SK-4105)
ImmPACT® DAB EqV Substrate Kit
(SK-4103-400)
ImmPACT® AEC Substrate Kit
(SK-4205)
ImmPACT® AMEC Red Substrate Kit
(SK-4285)
ImmPACT® VIP Substrate Kit
(SK-4605)
ImmPACT® SG Substrate Kit
(SK-4705)
ImmPACT® NovaRED Substrate Kit
(SK-4805)
DAB Substrate Kit
(SK-4100)
DAB Enhancing Solution
(H-2200-30)
Vector® VIP Substrate Kit
(SK-4600)
Vector® SG Substrate Kit
(SK-4700)
Vector® NovaRED Substrate Kit
(SK-4800)
AEC Substrate Kit
(SK-4200)
TMB Substrate Kit
(SK-4400)
ImmPACT® Vector® Red Substrate Kit
(SK-5105)
Vector® Red Substrate Kit
(SK-5100)
Vector® Blue Substrate Kit
(SK-5300)
Vector® Black Substrate Kit
(SK-5200)
BCIP/NBT Substrate Kit
(SK-5400)
Levamisole Solution
(SP-5000-18)
p-Nitrophenyl phosphate Substrate Kit
(SK-5900-18)
Blot Detection Systems
WestVision® Peroxidase Polymer Reagents for Western Blotting
(WB-1000-.8,WB-2000-.8)
In Situ Hybridization
Fluorescence Detection of Biotin-Labeled ISH Probes
(A-2011-1,BA-0300-.5)
Fluorescence Detection of Fluorescein-Labeled ISH Probes
(A-2011-1,BA-0601-.5)
In Situ Hybridization Detection Systems
(A-2011-1,BA-0300-.5,BA-0601-.5,H-1000-10,H-1200-10,H-1300-10,H-1400-10,H-1500-10,H-5000-60,H-5501-60,MB-1220-100,SA-5100-1,SK-5400,SP-5000-18,H-1700,H-1800)
Amplification of FISH using Biotinylated Anti-Avidin
(BA-0300-.5)
Biotin Labeling Reagents
Biotin (Long Arm) NHS
(SP-1200-50)
Biotin (Long Arm) NHS, water soluble
(SP-1210-50)
Biotin (Long Arm) Maleimide
(SP-1501-12)
Biotin (Long Arm) Hydrazide
(SP-1100-50)
Biotin Quantitation Kit
Quant*Tag Biotin Kit
(BDK-2000)
Thiol-Reactive Labeling Reagents
Biotin Maleimide
(SP-1501-12)
Fluorescein Maleimide
(SP-1502-12)
Texas Red Maleimide
(SP-1505-3.6)
Molecular Biology Reagents
5' EndTag Labeling System
(MB-9001)
3' EndTag Labeling System
(MB-9002)
PHOTOPROBE® Biotin
(SP-1000-.5,SP-1020-.5)
Detection of Labeled Nucleic Acid Probes on Blots
(H-3401-500,H-3402-500,H-3403-500,H-3404-100,SK-4103-400,SK-4105,SK-4200,SK-4205,SK-4285,SK-4600,SK-4605,SK-4700,SK-4705,SK-4800,SK-4805,SK-5100,SK-5105,SK-5200,SK-5300,SK-5400,SK-4100,SK-4400)
NicKit® Probe Size Optimization Kit
(MB-1905)
Lectins
Lectins in Histochemistry, ELISA, and Western Blot Applications
(AL-1013-2,AL-1063-2,AL-1143-2,AL-1173-2,AL-1183-2,AL-1213-2,AL-1233-2,AL-1303-2,AL-1353-2,AL-1393-2,B-1005-5,B-1005S-5,B-1015-5,B-1025-5,B-1025S-5,B-1035-5,B-1045-5,B-1055-5,B-1065-2,B-1075-5,B-1085-5,B-1105-2,B-1115-2,B-1125-2,B-1145-5,B-1155-5,B-1165-2,B-1175-1,B-1185-2,B-1205-.5,B-1215-2,B-1235-2,B-1245-2,B-1265-1,B-1305-2,B-1315-2,B-1325-2,B-1345-2,B-1355-2,B-1375-2,B-1385-2,B-1395-1,BK-1000,BK-3000,DL-1174-1,DL-1177-1,DL-1207-.5,FL-1001-25,FL-1011-2,FL-1021S-5,FL-1041-5,FL-1081-5,FL-1111-2,FL-1121-2,FL-1141-5,FL-1151-5,FL-1161-2,FL-1171-1,FL-1181-2,FL-1201-.5,FL-1211-2,FL-1231-2,FL-1241-2,FL-1301-2,FL-1311-2,FL-1321-2,FL-1341-2,FL-1351-2,FL-1391-1,FLK-2100,FLK-4100,L-1000-500,L-1020S-10,L-1050-10,L-1080-10,L-1090-10,L-1100-5,L-1110-5,L-1120-5,L-1140-10,L-1160-5,L-1170-2,L-1180-5,L-1190-1,L-1210-5,L-1230-5,L-1240-5,L-1260-2,L-1280-5,L-1290-1,L-1300-5,L-1310-5,L-1320-5,L-1340-5,L-1350-5,L-1370-5,L-1380-5,L-1390-2,L-1104-1,L-1410-5,DL-1178-1,AL-1003,AL-1023,AL-1023S,AL-1043,AL-1073,AL-1083,AL-1153,AL-1243,FL-1021,FL-1031,FL-1061,FL-1071,FL-1101,L-1010,L-1020,L-1030,L-1040,L-1060,L-1070,L-1150,DL-1048-1,L-1420-2,DL-1208-.5,DL-1067-1,DL-1068-1,CL-1003-1,CL-1073-1,CL-1075-1,CL-1303-1,CL-1305-1,BK-2100)
Agarose WGA - Use and Regeneration
(AL-1023)
Enrichment of NK cells by panning on ECL plates
(L-1140-10)
Specificity of Jacalin binding
(L-1150)