Slide Preparation

Useful tools for convenient slide preparation

Use the following accessories in slide preparation in IHC, ICC, in situ hybridization, and IF applications.

ImmEdge Hydrophobic Barrier PAP Pen

An improvement over the PAP pen, the ImmEdge pen provides a heat-stable, water-repellent barrier that keeps reagents localized on tissue specimens. The pen also prevents mixing of reagents when multiple sections are mounted on the same slide.

  • Stable for use with buffers with and without detergent (Tween 20, Triton X-100, etc.)
  • Light blue barrier compatible with enzyme or fluorescence-based detection systems
  • Soluble in all commonly used clearing agents 
  • Environmentally friendly - does not contain "ozone-depleting" substances as identified by the Montreal Protocol or the Council of the European Union

ImmPrint™ Histology Pen

You can use this solvent-resistant permanent marking pen for writing on frosted glass microscope slides, tissue cassettes, and most hard surfaces.

  • High-density, fast-drying, black ink is resistant to most organic solvents, such as alcohols and clearing agents
  • Smooth writing tip that resists drying out


You can use VECTABOND Reagent to adhere both frozen and paraffin-embedded tissue sections and cell preparations to glass slides and coverslips.

  • Tissue sections remain attached even when subjected to the most extreme conditions, such as high temperature antigen unmasking techniques and in situ hybridization
  • Chemically modifies the glass to form a highly adherent surface
  • Provided as 7 ml of concentrate that dilutes to 350 ml of treatment solution, sufficient for at least 500 standard slides



Deparaffinization & Rehydration

Before paraffin sections can be stained in an immunohistochemical (IHC) procedure, your slides need to be deparaffinized and rehydrated. This tutorial will show you how to remove the wax (paraffin) with xylene or xylene substitute, then rehydrate your sections through a graded alcohol series.