Lectins & Glycobiology Reagents

High-quality, purified lectins and other reagents for glycobiology and other applications

Lectins are sugar-binding proteins found in plants, viruses, microorganisms, and animals. They bind glycoconjugates in much the same way as antibodies bind antigens. As a result, researchers use lectins to explore a myriad of biological structures and processes.

Lectins bind specific carbohydrate structures, so you can use them in glycobiology to:

  • Separate oligosaccharides, even those with identical sugar compositions
  • Discriminate between oligosaccharide structures
  • Isolate a specific glycoconjugate, cell, or virus from a mixture

Some lectins can induce mitosis, so you can use them to investigate the process of lymphocyte blastogenesis and the biochemical and structural alterations associated with mitogenesis. These important applications include lymphokine production and viral coat protein (eg gp120) isolation.  

 

 

 

Vector Laboratories — a leader in lectins and other glycobiology products

At Vector Laboratories, we employ crystallization, precipitation, and chromatographic procedures as well as affinity chromatography to purify our lectins. We monitor the purification process carefully and assess the final product through:

  • Immunoprecipitation with antisera
  • Agglutination titer
  • Polyacrylamide gel electrophoresis
  • Binding activity to specific affinity columns

We do this to ensure you have the best lectins available on the market.

 

Validation data for Vector's Lectins

A selection of Vector Laboratories’ lectin products is validated by the National Center for Functional Glycomics (NCFG) using the Consortium for Functional Glycomics (CFG) glycan arrays. The NCFG analyzes the glycan specificity of each lectin lot and makes the data available to the public on the NCFG website.

 

Formalin-fixed, paraffin-embedded mouse kidney stained with Fl-AAL. Mounted with VECTASHIELD HardSet with DAPI.
Colon (FFPE): Antigen retrieved with Antigen Unmasking Solution, stained with Cy5 Sambucus Nigra Lectin (SNA; fuchsia). DAPI counterstain (blue).

Explore Lectins & Glycobiology Reagents

Lectin screening kits provide a panel of lectins or lectin conjugates selected to provide a variety of sugar binding preferences. The smaller volume unit sizes in these kits are of the same high quality as individually offered lectin units.

All lectins manufactured by Vector Laboratories are extracted from a native plant source (i.e. seeds, bulbs, fruit etc.) and are not recombinant. Lectins are grouped based on their monsaccharide binding preferences.

Anti-lectin antibodies are offered in unconjugated or in biotinylated formats. In some applications, using biotinylated anti-lectins in conjunction with unlabeled lectins may provide greater sensitivity than using biotinylated lectins alone.

While the eluting solutions are primarily used in lectin agarose column and slurry preparations, inhibiting sugars, when combined with lectin solutions, have appeal as negative controls to prevent binding in IHC, IF, ELISA and blotting applications.


Resources

Lectins and Glycobiology

This brochure contains a useful table of lectin properties summarizing biophysical information as well as sugar binding specificities and special applications for lectins available from Vector Labs. Also includes a complete product listing of all lectins, lectin conjugates and associated reagents.