Lectins

Highly purified, quality-controlled lectins for glycobiology and other applications

Specificity for complex carbohydrate groups and other unique properties have made lectins powerful tools for researchers. Vector Labs offers you over 150 lectin binding assays for:

  • Glycobiology
  • Immunohistochemistry (IHC)
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Blotting applications 
  • ELISAs and more

All lectins are highly purified and subjected to intensive quality control to ensure you have the best lectins available on the market. 

Lectin validation data from the NCFG

The National Center for Functional Glycomics (NCFG), with the support of the NIH Common Fund for Glycosciences, is screening a selection of Vector Labs's lectins. Using the Consortium for Functional Glycomics (CFG) glycan arrays, the NCFG analyzes the glycan specificity of each of our lectin lots, and provides these detailed lectin-glycan binding data to you on the NCFG website.

The goal of this NCFG project is to support rigorous and reproducible glycobiology experiments.

These data provide uniquely valuable insights into the glycan structures on the glycoproteins you are studying:

  • Binding specificity of each lectin, for each of the hundreds of glycan structures in the array
  • Data tabulated and conveniently summarized as a binding array, with peak height indicating degree of binding for each glycan 
  • Specific and detailed data on complex carbohydrate structure
  • Lot-specific data — for confidence in the reproducibility of your binding data
Retina flat mount from mice, perfused with DyLight 594-LEL. The retina were mounted in Vectashield HardSet+DAPI. Image courtesy of George W. Smith, Florida Atlantic University.
Mouse kidney, formalin-fixed, paraffin-embedded: DyLight 488 conjugated Lycopersicon esculentum lectin (green) mounted in VECTASHIELD HardSet Mounting Medium with DAPI.


Resources

Immunohistochemistry Resource Guides

The Immunohistochemistry Resource Guide will help you navigate through the many choices of detection systems, substrates, and ancillary reagents that complete your workflow.
Learn more about this guide >

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IHC Multiplexing Guide

A valuable resource for those considering localizing two or more antigens in the same tissue section. Protocols for chromogenic detection are accompanied by schematic illustrations of the procedures.
A chart of all pair-wise combinations of substrates is included along with photographic examples. Practical tips regarding order of substrates, blocking, and proper controls are also provided.

Immunofluorescence Resource Guide

Producing immunofluorescence (IF) staining results with high specificity and sensitivity begins with choosing the best reagents.  This guide will help you:

  • Learn about the IF workflow and how to optimize each step
  • Quickly identify and choose the most appropriate fluorescent secondary conjugates
  • Select the best anti-fade medium to preserve your fluorescence signal for imaging and archiving

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Deparaffinization & Rehydration

Before paraffin sections can be stained in an immunohistochemical (IHC) procedure, your slides need to be deparaffinized and rehydrated. This tutorial will show you how to remove the wax (paraffin) with xylene or xylene substitute, then rehydrate your sections through a graded alcohol series.