Autofluorescence Quenching Kits

Dramatically reduce autofluorescence to reveal true immunofluorescence

In tissue sections, autofluorescence is the unwanted fluorescence that can make it difficult or impossible to distinguish antigen-specific signal from non-specific background noise. The novel, patent-pending Vector TrueVIEW® Autofluorescence Quenching Kit specifically binds and quenches autofluorescent elements from non-lipofuscin sources, significantly enhancing signal-to-noise in most immunofluorescence assays—even in the most challenging tissues.

 

 

Use TrueVIEW Quencher if you need an autofluorescence blocking agent that:

  • Specifically reduces autofluorescence from non-lipofuscin sources
  • Is easy to use
  • Is compatible with a wide selection of fluorophores
  • Is compatible with standard epifluorescence and confocal laser microscopes

 

Comparison with other autofluorescence reducing agents

Whereas most methods for reducing tissue autofluorescence act primarily on lipofuscin granules, the Vector TrueVIEW® quencher targets fluorescence from non-lipofuscin sources, including aldehyde fixation, red blood cells, and structural elements, such as collagen and elastin. It provides a clear, unambiguous “true view” of target antigen localization, even in problematic tissues, such as kidney, spleen, and pancreas.

 

Comparisons with other commercial and “home brew” approaches show that Vector TrueVIEW is easier to use and more effective at reducing autofluorescence. The images below show the results of side-by-side comparisons on serial sections of formalin-fixed, paraffin embedded human pancreas visualized using a standard fluorescein (green) filter. No specific immunofluorescence staining was conducted.

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