Alkaline Phosphatase (AP) Substrates

Vector offers alkaline phosphatase (AP) substrates in both original and ImmPACT™ formulations.

The original Vector® AP substrates offer a range of color choices and the following advantages:

  • Allow you to stain antigens in pigmented tissues and melanomas
  • Enable localization of multiple antigens in a single tissue section (the combination of Vector® Red and Vector® Blue substrate, for example, are useful in co-localization experiments)
  • Vector® Red and Vector® Blue substrates can be viewed with both conventional light microscopy as well as fluorescence miscroscopy
  • Vector® AP substrates can be both non-aqueous and aqueously mounted with the exception of Vector® Black, which should only be non-aqueously mounted
  • Vector® Blue substrate generates a product that can be cleared with citrus oil-based clearing agents and then permanently mounted
  • The BCIP/NBT substrate is frequently used for nitrocellulose or in situ hybridization applications

Our newer ImmPACT™ Vector® Red Substrate is the most sensitive red AP substrate:

  • 2-4 fold greater sensitivity than the original substrate
  • Supplied with stock solutions in dropper bottles and optimized diluent
  • Optimized specifically for immunohistochemistry applications
  • Can be viewed with fluorescence as well as light microscopy