SpeakEasy Science Blog

The Sialylation Pathways in Pancreatic Cancer and Their Role in Tumor Microenvironment Differentiation

Hypersialylation of glycans is one of the key drivers in cell differentiation in cancer, including pancreatic ductal adenocarcinoma (PDAC) cells. In this article, we review a study investigating how this mechanism led to the differentiation of tumor monocytes into malignant tumor macrophages associated with low survival rates in patients.
July 18, 2021

The Life of a Histotechnologist: One Thin Section

Eric Peterson has long held an interest in the underpinnings of life, found enjoyment in science fiction, and taken a practical approach to career choices rooted in economics. He is not the guy who plotted his career path from start-to-finish; instead, he chose a career in the growing field of technology and was confident that there was a stable market for scientific professionals.
July 12, 2021

Investigating Cross-Reactive Antibodies of SARS-CoV-2 with LIBRA-seq

In this publication highlight, we review the use of LIBRA-seq to characterize monoclonal antibodies from a recovered SARS-CoV donor, expanding our understanding of cross-reactive antibodies for coronavirus.
July 9, 2021

Dial up the Contrast with Counterstaining

When scientists detect targets of interest with immunohistochemistry (IHC), antibody binding and substrate detection alone often produce a perplexing picture where the target lacks context. Luckily there’s a solution. Counterstaining IHC samples after enzymatic detection spatially orients the target within its surroundings, which can help guide researchers as they analyze their results.
July 1, 2021

Macrophage-derived Microparticles to Improve the Efficacy of Anti-PD-1 Antibodies in Tumors

In this publication highlight, we review a study examining the impact of macrophage-derived microparticles on the tumor microenvironment and their synergistic effect on anti-PD-1 antibody activity.]
June 29, 2021

Getting to the Specifics: Blocking for Immunohistochemistry

When performing IHC, scientists aspire to reduce background or non-specific staining on the specimen. Explore how you can block the sources of non-specific signals increases the signal-to-noise ratio for optimal results.

June 21, 2021

An Introduction to Bioconjugation: Answering Your Questions

Bioconjugation offers a way to chemically link two molecules to form a single hybrid, where at least one of the molecules in the partnership is a biomolecule. The resulting product retains the activity of each component, yet also gains a novel function that is not possible with either molecule alone. In a recent webinar, Dr. Craig Pow, Director, Technical Services at Vector Laboratories, provided an introduction to bioconjugation and its applications, as well as key factors to success.
June 8, 2021

Better Immunofluorescence at the Speed of Light

When everything goes right, looking through the microscope at your immunofluorescence-stained tissue is no less beautiful than peering through a telescope at the night sky. Unfortunately, experimental issues can obscure that pristine view of your microscopic “galaxy” so much that that you can’t tell the difference between Halley’s Comet and the Big Dipper! Never fear, cellgazer: we can help!
June 2, 2021

Improving IHC & IF Staining Results

Immunohistochemistry (IHC) and immunofluorescence (IF) are widely used across the life sciences—in cancer research, immunology, neuroscience, cell biology, and histopathology—to help elucidate protein expression in cell and tissue specimens.
May 7, 2021